Subtopic Deep Dive
Dihydromyricetin Antioxidant Activity
Research Guide
What is Dihydromyricetin Antioxidant Activity?
Dihydromyricetin antioxidant activity refers to the free radical scavenging and ROS-reducing properties of dihydromyricetin (DHM), a flavonoid from Ampelopsis grossedentata, studied in oxidative stress models and disease contexts.
DHM demonstrates antioxidant effects by modulating Nrf2 signaling, reducing reactive oxygen species (ROS), and enhancing redox homeostasis (Hu et al., 2017; Zeng et al., 2018). Research spans in vitro cell models, animal studies, and extraction optimization from plant sources, with over 20 papers in the provided lists. Key mechanisms include apoptosis induction via ROS modulation in cancer cells (Zhou et al., 2014; Liu et al., 2014).
Why It Matters
DHM's antioxidant activity protects against oxidative damage in NAFLD by improving mitochondrial function (Zeng et al., 2018, 160 citations), suppresses NLRP3 inflammasome in endothelial cells (Hu et al., 2017, 176 citations), and induces apoptosis in hepatocellular carcinoma via ROS reduction (Liu et al., 2014, 89 citations). These effects support natural therapeutics for chronic inflammation, liver disease, and neurodegeneration (Arulselvan et al., 2016, 949 citations; Ren et al., 2016, 97 citations). Applications include optimized extraction for supplements (Liao et al., 2014, 55 citations; Wang et al., 2011, 38 citations).
Key Research Challenges
Structure-Activity Relationships
Defining precise molecular interactions of DHM with ROS and enzymes like SOD remains unclear across models. Studies show flavonoid structure influences efficacy but lack comprehensive SAR mapping (Zhou et al., 2014). In vivo translation requires better pharmacokinetic data (Zeng et al., 2018).
In Vivo Oxidative Stress Models
Translating cell-based ROS reduction to animal disease models faces variability in bioavailability. MPTP Parkinson's models show neuroprotection but need dose optimization (Ren et al., 2016). NAFLD studies highlight mitochondrial benefits yet struggle with long-term efficacy (Zeng et al., 2018).
Extraction and Purity Optimization
Achieving high-purity DHM from plants via recrystallization or SC-CO2 extraction impacts activity consistency. Methods yield 98% purity but scale-up challenges persist (Liao et al., 2014; Wang et al., 2011). Linking purity to bioactivity needs standardized assays.
Essential Papers
Role of Antioxidants and Natural Products in Inflammation
Palanisamy Arulselvan, Masoumeh Tangestani Fard, Woan Sean Tan et al. · 2016 · Oxidative Medicine and Cellular Longevity · 949 citations
Inflammation is a comprehensive array of physiological response to a foreign organism, including human pathogens, dust particles, and viruses. Inflammations are mainly divided into acute and chroni...
Dihydromyricetin inhibits NLRP3 inflammasome‐dependent pyroptosis by activating the Nrf2 signaling pathway in vascular endothelial cells
Qin Hu, Ting Zhang, Long Yi et al. · 2017 · BioFactors · 176 citations
Abstract Increasing evidence demonstrates that pyroptosis, pro‐inflammatory programmed cell death, is linked to atherosclerosis; however, the underlying mechanisms remain to be elucidated. Dihydrom...
Dihydromyricetin Ameliorates Nonalcoholic Fatty Liver Disease by Improving Mitochondrial Respiratory Capacity and Redox Homeostasis Through Modulation of SIRT3 Signaling
Xianglong Zeng, Jining Yang, Ou Hu et al. · 2018 · Antioxidants and Redox Signaling · 160 citations
<b><i>Aims:</i></b> Our previous clinical trial indicated that the flavonoid dihydromyricetin (DHM) could improve hepatic steatosis in patients with nonalcoholic fatty liver disease (NAFLD), altoug...
Ampelopsin Induces Cell Growth Inhibition and Apoptosis in Breast Cancer Cells through ROS Generation and Endoplasmic Reticulum Stress Pathway
Yong Zhou, Furong Shu, Xinyu Liang et al. · 2014 · PLoS ONE · 107 citations
Ampelopsin (AMP), a major bioactive constituent of Ampelopsis grossedentata, exerts a number of biological effects. In this study, we investigated its anti-cancer activity in human breast cancer ce...
The Versatile Effects of Dihydromyricetin in Health
LI Hong-liang, Qisheng Li, Zhaowen Liu et al. · 2017 · Evidence-based Complementary and Alternative Medicine · 98 citations
Dihydromyricetin is a flavonoid isolated from Ampelopsis grossedentata , which is traditionally used in China. Dihydromyricetin exhibits health‐benefiting activities with minimum adverse effects. D...
Dihydromyricetin protects neurons in an MPTP-induced model of Parkinson's disease by suppressing glycogen synthase kinase-3 beta activity
Zhaoxiang Ren, Yafei Zhao, Ting Cao et al. · 2016 · Acta Pharmacologica Sinica · 97 citations
Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of <i>Alternanthera sessilis</i> via the Inhibition of the NF-<i>κ</i>B Pathway
Katyakyini Muniandy, Sivapragasam Gothai, Khaleel M. H. Badran et al. · 2018 · Journal of Immunology Research · 90 citations
Alternanthera sessilis , an edible succulent herb, has been widely used as herbal drug in many regions around the globe. Inflammation is a natural process of the innate immune system, accompanied w...
Reading Guide
Foundational Papers
Start with Zhou et al. (2014, 107 cites) for ROS-mediated apoptosis in cancer and Liu et al. (2014, 89 cites) for HCC mechanisms, as they establish core DHM-ROS links; add Liao et al. (2014, 55 cites) for extraction baselines.
Recent Advances
Study Zeng et al. (2018, 160 cites) for SIRT3 in NAFLD and Hu et al. (2017, 176 cites) for Nrf2 in pyroptosis, capturing in vivo advances.
Core Methods
Core techniques: DPPH/ABTS scavenging assays, DCFH-DA ROS probes, Nrf2 luciferase reporters, SC-CO2 extraction, and Western blots for SIRT3/GSK-3β (Wang et al., 2011; Ren et al., 2016).
How PapersFlow Helps You Research Dihydromyricetin Antioxidant Activity
Discover & Search
Research Agent uses searchPapers and exaSearch to find DHM antioxidant papers like 'Dihydromyricetin Ameliorates Nonalcoholic Fatty Liver Disease' (Zeng et al., 2018), then citationGraph reveals 160 citing works on redox signaling, while findSimilarPapers uncovers related flavonoids in inflammation (Arulselvan et al., 2016).
Analyze & Verify
Analysis Agent applies readPaperContent to extract Nrf2 pathway details from Hu et al. (2017), verifies claims with CoVe against 10+ papers for ROS reduction consistency, and uses runPythonAnalysis to plot dose-response curves from extracted data with matplotlib, graded via GRADE for evidence strength in vivo models.
Synthesize & Write
Synthesis Agent detects gaps in SAR studies across papers, flags contradictions in ROS induction vs. reduction (Zhou et al., 2014 vs. Liu et al., 2014), and Writing Agent employs latexEditText for mechanism diagrams, latexSyncCitations for 20+ refs, and latexCompile for publication-ready reviews with exportMermaid flowcharts of signaling pathways.
Use Cases
"Extract and plot ROS reduction data from DHM papers in cancer models."
Research Agent → searchPapers('DHM ROS cancer') → Analysis Agent → readPaperContent(Zhou 2014, Liu 2014) → runPythonAnalysis(pandas plot dose-response curves) → matplotlib figure of IC50 values.
"Draft LaTeX review on DHM extraction methods and antioxidant activity."
Research Agent → citationGraph(Liao 2014) → Synthesis Agent → gap detection → Writing Agent → latexEditText(structure review) → latexSyncCitations(5 extraction papers) → latexCompile(PDF with purity-activity table).
"Find GitHub repos analyzing DHM metabolomics data."
Research Agent → searchPapers('DHM metabolomics') → Code Discovery → paperExtractUrls(Le 2016) → paperFindGithubRepo → githubRepoInspect(insulin sensitivity scripts) → runPythonAnalysis(reproduce glucose homeostasis plots).
Automated Workflows
Deep Research workflow scans 50+ DHM papers via searchPapers → citationGraph → structured report on antioxidant mechanisms with GRADE scores. DeepScan applies 7-step CoVe to verify Nrf2 claims in Hu et al. (2017), checkpointing extraction → analysis → synthesis. Theorizer generates hypotheses on DHM-SIRT3 interactions from Zeng et al. (2018) data.
Frequently Asked Questions
What is dihydromyricetin antioxidant activity?
DHM exhibits free radical scavenging and ROS reduction, modulating Nrf2 and SIRT3 pathways (Hu et al., 2017; Zeng et al., 2018).
What are key methods for studying DHM antioxidants?
Methods include cell apoptosis assays, MPTP mouse models, SC-CO2 extraction, and ROS quantification (Zhou et al., 2014; Ren et al., 2016; Wang et al., 2011).
What are top papers on DHM antioxidant activity?
High-citation works: Zeng et al. (2018, 160 cites, NAFLD redox), Hu et al. (2017, 176 cites, NLRP3), Liu et al. (2014, 89 cites, HCC ROS).
What open problems exist in DHM research?
Challenges include SAR mapping, in vivo bioavailability, and purity-activity links (Liao et al., 2014; Zeng et al., 2018).
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