Subtopic Deep Dive

Fungicide Resistance Management Strategies
Research Guide

What is Fungicide Resistance Management Strategies?

Fungicide resistance management strategies are integrated approaches including fungicide rotation, mixtures, and IPM to delay resistance development in fungal plant pathogens like Botrytis cinerea.

These strategies counter resistance evolution observed in field populations of grey mould fungi. Key studies document multidrug resistance mechanisms and evaluate control tactics (Hahn, 2014; Kretschmer et al., 2009). Over 400 papers address Botrytis resistance since 2007, with Williamson et al. (2007) cited 1615 times.

15
Curated Papers
3
Key Challenges

Why It Matters

Fungicide resistance shortens product lifespans, raising crop loss risks in strawberries and grapes; Hahn (2014) details Botrytis cases with 493 citations. Rotation and mixtures extend efficacy, as shown in field trials by Kretschmer et al. (2009, 401 citations). IPM integration with biofungicides reduces reliance on synthetics (Abbey et al., 2018, 241 citations), sustaining yields amid 24% postharvest losses (Wisniewski & Wilson, 1992, 287 citations).

Key Research Challenges

Multidrug Resistance Evolution

Field strains of Botrytis cinerea develop resistance to multiple fungicide classes via target mutations. Kretschmer et al. (2009) identify mechanisms in 401-cited study. Management requires monitoring shifts in resistance profiles (Leroux et al., 2010).

Resistance Monitoring in Fields

Detecting low-frequency resistant mutants demands sensitive assays across crops. Hahn (2014) highlights threats in Botrytis with 493 citations. Field trials struggle with variability (Petrasch et al., 2019).

Balancing IPM and Fungicides

Integrating biocontrol with chemicals faces efficacy gaps in humid conditions. Abbey et al. (2018) review biofungicide challenges (241 citations). Optimizing mixtures avoids fitness costs in resistant strains (Leroch et al., 2010).

Essential Papers

1.

<i>Botrytis cinerea</i> : the cause of grey mould disease

B. Williamson, Bettina Tudzynski, Paul Tudzynski et al. · 2007 · Molecular Plant Pathology · 1.6K citations

SUMMARY Introduction: Botrytis cinerea (teleomorph: Botryotinia fuckeliana ) is an airborne plant pathogen with a necrotrophic lifestyle attacking over 200 crop hosts worldwide. Although there are ...

2.

Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea

Joëlle Amselem, Christina A. Cuomo, J.A.L. van Kan et al. · 2011 · PLoS Genetics · 1.1K citations

Sclerotinia sclerotiorum and Botrytis cinerea are closely related necrotrophic plant pathogenic fungi notable for their wide host ranges and environmental persistence. These attributes have made th...

3.

The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study

Matthias Hahn · 2014 · Journal of Chemical Biology · 493 citations

4.

Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen <i>Botrytis cinerea</i>

Stefan Petrasch, Steven J. Knapp, J.A.L. van Kan et al. · 2019 · Molecular Plant Pathology · 441 citations

Summary The fungal pathogen Botrytis cinerea causes grey mould, a commercially damaging disease of strawberry. This pathogen affects fruit in the field, storage, transport and market. The presence ...

5.

Fungicide-Driven Evolution and Molecular Basis of Multidrug Resistance in Field Populations of the Grey Mould Fungus Botrytis cinerea

Matthias Kretschmer, Michaela Leroch, Andreas Mosbach et al. · 2009 · PLoS Pathogens · 401 citations

The grey mould fungus Botrytis cinerea causes losses of commercially important fruits, vegetables and ornamentals worldwide. Fungicide treatments are effective for disease control, but bear the ris...

6.

Biological Control of Postharvest Diseases of Fruits and Vegetables: Recent Advances

Michael Wisniewski, Charles L. Wilson · 1992 · HortScience · 287 citations

Although it is difficult to determine the full extent of postharvest losses due to diseases, conservative estimates place losses to fruits and vegetables from spoilage at ≈24% of the harvested crop...

7.

Exploring Mechanisms of Resistance to Respiratory Inhibitors in Field Strains of <i>Botrytis cinerea</i> , the Causal Agent of Gray Mold

Pierre Leroux, Michel Gredt, Michaela Leroch et al. · 2010 · Applied and Environmental Microbiology · 269 citations

ABSTRACT Respiratory inhibitors are among the fungicides most widely used for disease control on crops. Most are strobilurins and carboxamides, inhibiting the cytochrome b of mitochondrial complex ...

Reading Guide

Foundational Papers

Start with Williamson et al. (2007, 1615 citations) for Botrytis fungicide context, then Kretschmer et al. (2009, 401 citations) for resistance mechanisms, and Hahn (2014, 493 citations) for management threats.

Recent Advances

Study Petrasch et al. (2019, 441 citations) on strawberry grey mould and Abbey et al. (2018, 241 citations) on biofungicides.

Core Methods

Resistance monitoring via EC50 assays (Leroux et al., 2010); genomic analysis of mutants (Amselem et al., 2011); field rotation trials (Kretschmer et al., 2009).

How PapersFlow Helps You Research Fungicide Resistance Management Strategies

Discover & Search

Research Agent uses searchPapers and exaSearch to find 50+ papers on Botrytis resistance, then citationGraph on Kretschmer et al. (2009) reveals 401-cited networks linking Hahn (2014). findSimilarPapers expands to Sclerotinia strategies.

Analyze & Verify

Analysis Agent applies readPaperContent to extract resistance mechanisms from Leroux et al. (2010), verifies claims with CoVe against Williamson et al. (2007), and runs PythonAnalysis on dosage-response data for EC50 stats using pandas. GRADE scores evidence on rotation efficacy.

Synthesize & Write

Synthesis Agent detects gaps in biofungicide IPM via contradiction flagging across Abbey et al. (2018) and Hahn (2014); Writing Agent uses latexEditText for strategy tables, latexSyncCitations for 10+ refs, and latexCompile for reports with exportMermaid timelines of resistance evolution.

Use Cases

"Analyze resistance frequency trends in Botrytis field trials from 2009-2019"

Research Agent → searchPapers('Botrytis resistance field trials') → Analysis Agent → runPythonAnalysis(pandas plot of frequencies from Leroux et al. 2010 + Kretschmer et al. 2009) → matplotlib trend graph.

"Draft LaTeX review on fungicide rotation for strawberry grey mould"

Synthesis Agent → gap detection(Hahn 2014, Petrasch 2019) → Writing Agent → latexEditText(structure) → latexSyncCitations(Williamson 2007 et al.) → latexCompile(PDF with tables).

"Find code for simulating fungicide resistance evolution models"

Research Agent → paperExtractUrls(Hahn 2014) → Code Discovery → paperFindGithubRepo → githubRepoInspect → Python model from Botrytis sim repo.

Automated Workflows

Deep Research workflow scans 50+ papers via searchPapers on 'Botrytis resistance management', structures report with IPM gaps graded by Analysis Agent. DeepScan applies 7-step CoVe to verify rotation efficacy in Kretschmer et al. (2009) against field data. Theorizer generates hypotheses on biofungicide mixtures from Abbey et al. (2018) + genomic insights (Amselem et al., 2011).

Frequently Asked Questions

What defines fungicide resistance management?

Integrated tactics like rotation, mixtures, and IPM delay resistance in pathogens such as Botrytis cinerea (Hahn, 2014).

What are common methods?

Product rotation avoids selection; mixtures suppress mutants; biofungicides integrate via IPM (Kretschmer et al., 2009; Abbey et al., 2018).

What are key papers?

Williamson et al. (2007, 1615 citations) on Botrytis biology; Kretschmer et al. (2009, 401 citations) on multidrug resistance; Hahn (2014, 493 citations) on threats.

What open problems exist?

Predicting resistance spread in diverse fields; scaling biofungicides; modeling multi-class resistance fitness (Leroux et al., 2010; Petrasch et al., 2019).

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